Multiplexed temporal quantification of the exercise-regulated plasma peptidome

BL Parker; JG Burchfield; D Clayton; TA Geddes; RJ Payne; B Kiens; JFP Wojtaszewski; EA Richter; DE James

Journal article

Multiplexed temporal quantification of the exercise-regulated plasma peptidome

BL Parker, JG Burchfield, D Clayton, TA Geddes, RJ Payne, B Kiens, JFP Wojtaszewski, EA Richter, DE James

Molecular and Cellular Proteomics | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2017

DOI: 10.1074/mcp.RA117.000020

Abstract

Exercise is extremely beneficial to whole body health reducing the risk of a number of chronic human diseases. Some of these physiological benefits appear to be mediated via the secretion of peptide/protein hormones into the blood stream. The plasma peptidome contains the entire complement of low molecular weight endogenous peptides derived from secretion, protease activity and PTMs, and is a rich source of hormones. In the current study we have quantified the effects of intense exercise on the plasma peptidome to identify novel exercise regulated secretory factors in humans. We developed an optimized 2D-LC-MS/MS method and used multiple fragmentation methods including HCD and EThcD to analy..

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University of Melbourne Researchers

Ben Parker Author

Related Projects (1)

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Grants

Funding Acknowledgements

This work was supported by a National Health and Medical Research Council (NHMRC) project grant (D.E.J and B.L.P) and by the Danish Research Council for Independent Research/Medicine (E.A.R.). B.L.P is a recipient of an NHMRC Early Career Fellowship. D.E.J. is a recipient of an NHRMC Senior Research Fellowship. The contents of the published material are solely the responsibility of the individual authors and do not reflect the view of NHMRC.